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Antibodies sure to the blocking peptide no more bind to the epitope to the focus on protein. By evaluating the staining with the blocked antibody compared to the antibody alone, you can see which staining is particular.
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This guideline outlines more doable optimization of cellular and immunoassay parameters to ensure the very best final results are...
This tutorial outlines further more feasible optimization of mobile and immunoassay parameters to make certain the very best success are...
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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-get together scientific information. Neither the written content nor the BenchSci technological know-how and procedures for range are actually evaluated by us; we are providing them as-is and without guarantee of any kind, including for use or software of your Thermo Fisher Scientific products and solutions introduced.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both of those antibodies...
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Blocking peptides are peptides that bind precisely for the concentrate on antibody and block antibody binding. These peptide generally is made up of the epitope identified because of the antibody.
Goal more info Info Cyclin E belongs to the remarkably conserved cyclin family, whose associates are characterized by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins exhibit unique expression and degradation designs which contribute towards the temporal coordination of each mitotic party. Cyclin E varieties a complex with and capabilities being a regulatory subunit of CDK2, whose action is needed for cell cycle G1/S transition. This protein accumulates on the G1-S period boundary and is degraded as cells progress through S section.
Overexpression of the gene has long been observed in several tumors, which results in chromosome instability, and therefore may perhaps add to tumorigenesis. This protein was located to affiliate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle regulated histone gene expression and plays a critical function in selling mobile-cycle development in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are explained. Two more splice variants were reported but detailed nucleotide sequence information isn't however obtainable.
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